8TXT
Crystal structure of 05.GC.w13.02 Fab in complex with H5 HA from A/Viet Nam/1203/2004(H5N1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97934 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 159.461, 232.604, 233.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.120 - 3.190 |
R-factor | 0.2196 |
Rwork | 0.217 |
R-free | 0.25830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fqi |
RMSD bond length | 0.002 |
RMSD bond angle | 0.484 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.120 | 3.300 |
High resolution limit [Å] | 3.190 | 3.200 |
Rpim | 0.096 | 0.800 |
Number of reflections | 57122 | 689 |
<I/σ(I)> | 7.8 | |
Completeness [%] | 96.6 | |
Redundancy | 5.8 | |
CC(1/2) | 0.976 | 0.360 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M lithium sulfate, pH 6.2, 20% PEG3350 |