8TWP
Influenza A virus (A/Aichi/2/1968(H3N2) nucleoprotein mutant - 2-7 deleted, R416A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-21 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 163.840, 282.920, 116.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.680 - 2.900 |
| R-factor | 0.2207 |
| Rwork | 0.220 |
| R-free | 0.24450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.557 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 59023 | 4122 |
| <I/σ(I)> | 15.71 | |
| Completeness [%] | 98.1 | |
| Redundancy | 5.5 | |
| CC(1/2) | 0.999 | 0.646 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES pH 8.0, 1.33 % (w/v) polyethylene glycol monomethyl ether 2000, 0.9 M potassium sodium tartrate |
