8TWO

AvrB bound with UDP and RIN4_T166-Rha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0358
Spacegroup name	P 21 21 2
Unit cell lengths	57.990, 119.236, 39.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.150 - 2.090
R-factor	0.2275
Rwork	0.221
R-free	0.28990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.554
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.150	2.130
High resolution limit [Å]	2.090	2.090
Number of reflections	16986	828
<I/σ(I)>	6.61
Completeness [%]	99.9
Redundancy	6.3
CC(1/2)	0.997	0.233

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM Tris (pH 7.5~7.8), 27%~32% PEG 550 MME