8TWG

Crystal structure of tetracycline destructase Tet(56-2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 2 21 21
Unit cell lengths	46.790, 70.548, 128.314
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.970 - 1.800
R-factor	0.1761
Rwork	0.174
R-free	0.20950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.877
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.970	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	40189	3916
<I/σ(I)>	17.78
Completeness [%]	99.8
Redundancy	6.51
CC(1/2)	0.990	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.15 M Magnesium formate, 15% Polyethylene glycol