8TWD
Structure of IraM-bound RssB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-02 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.977 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 130.664, 191.229, 96.985 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.330 - 3.300 |
| R-factor | 0.2474 |
| Rwork | 0.245 |
| R-free | 0.27060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.643 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.330 | 3.560 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rpim | 0.038 | |
| Number of reflections | 18674 | 3782 |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.2 | |
| CC(1/2) | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1M sodium acetate pH 4.5 120mM magnesium acetate |
