8TV2
Structure of apo FabS1C_L1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.259, 74.259, 370.837 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.310 - 2.650 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.24730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.197 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.710 | 2.780 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.128 | 1.136 |
| Rmeas | 0.136 | 1.209 |
| Rpim | 0.045 | 0.405 |
| Number of reflections | 33902 | 3816 |
| <I/σ(I)> | 9.3 | 1.9 |
| Completeness [%] | 94.1 | 82.3 |
| Redundancy | 8.9 | 8.7 |
| CC(1/2) | 0.994 | 0.702 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 18% PEG6000, 1M Lithium Chloride, 100 mM Sodium citrate 5.0 |
