8TUS

WU Polyomavirus LTA NLS bound to importin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 21 21 21
Unit cell lengths	78.401, 89.514, 102.329
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.530 - 2.600
R-factor	0.2133
Rwork	0.212
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.502
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.530	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.160	0.660
Rmeas	0.170	0.720
Rpim	0.070	0.280
Number of reflections	22596	2746
<I/σ(I)>	5.7	1.8
Completeness [%]	99.5
Redundancy	5.7
CC(1/2)	0.980	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.75 M sodium citrate, 0.1 M HEPES pH 7.0, 0.01 M DTT