8TTV
Structure of SNX27 FERM complexed with Fam21A repeat 20 (1289-1302)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953700 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.965, 74.200, 105.189 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.910 - 2.000 |
| R-factor | 0.2124 |
| Rwork | 0.207 |
| R-free | 0.26430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.009 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.910 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.240 | 2.161 |
| Rmeas | 0.260 | 2.366 |
| Rpim | 0.101 | 0.943 |
| Total number of observations | 139518 | 8637 |
| Number of reflections | 20806 | 1385 |
| <I/σ(I)> | 6.7 | 1 |
| Completeness [%] | 99.5 | |
| Redundancy | 6.7 | 6.2 |
| CC(1/2) | 0.989 | 0.205 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Sodium Cacodylate pH 6.5 and 22.5% PEG4000 |
