8TTC
Structure of retromer VPS29-VPS35 (483-796) complexed with Fam21A repeat 20 (1289-1302)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-13 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.95365 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 88.763, 88.763, 328.291 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.580 - 3.010 |
R-factor | 0.2393 |
Rwork | 0.238 |
R-free | 0.27050 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.082 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.900 | 3.200 |
High resolution limit [Å] | 3.010 | 3.010 |
Rmerge | 0.137 | 1.588 |
Rmeas | 0.143 | 1.652 |
Rpim | 0.040 | 0.451 |
Total number of observations | 348827 | 54935 |
Number of reflections | 27047 | 4201 |
<I/σ(I)> | 11.7 | 1.3 |
Completeness [%] | 99.6 | |
Redundancy | 12.9 | 13.1 |
CC(1/2) | 0.998 | 0.812 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 0.1 M Sodium Citrate pH 4.2, 0.2 M NaCl 16% PEG1000 |