8TRS

Structure of the EphA2 CRD bound to FabS1CE_C1, trigonal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	71.376, 71.376, 235.014
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.810 - 1.900
R-factor	0.182
Rwork	0.180
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fl7
RMSD bond length	0.007
RMSD bond angle	0.959
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	117.510	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.050	1.516
Rmeas	0.052	1.585
Rpim	0.015	0.458
Number of reflections	60648	3633
<I/σ(I)>	28.7	1.6
Completeness [%]	100.0	99.3
Redundancy	11.5
CC(1/2)	1.000	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	200 mM ammonium chloride, 30% PEG600 (GRAS1 condition A8)