8TRI

Crystal Structure of Mouse Cadherin-23 EC25-MAD28 F2894A

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	146.994, 164.657, 183.634
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.563 - 3.716
Rwork	0.187
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.622
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.760
High resolution limit [Å]	3.700	3.700
Number of reflections	47174	2309
<I/σ(I)>	11.5	1.96
Completeness [%]	99.9	99.6
Redundancy	10.4	7
CC(1/2)	0.981	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	277	1.25 M Lithium Acetate, 0.1 M MES pH 7.2, 25% glycerol (cryo)