8TQ7
Crystal structure of Fab.34.2.12 in complex with MHC-I (H2-Dd)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 273 |
Detector technology | PIXEL |
Collection date | 2022-09-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.587, 113.326, 196.369 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.620 - 2.800 |
R-factor | 0.2272 |
Rwork | 0.225 |
R-free | 0.27060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5weu |
RMSD bond length | 0.004 |
RMSD bond angle | 0.680 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.620 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.099 | 1.320 |
Rpim | 0.041 | 0.547 |
Number of reflections | 48825 | 4505 |
<I/σ(I)> | 12.9 | 1.2 |
Completeness [%] | 96.9 | 93.5 |
Redundancy | 6.7 | 6.7 |
CC(1/2) | 0.998 | 0.528 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 12% PEG8000, 0.1 M MES, pH 6.5 |