8TPK

P6522 crystal form of C. crescentus DriD-ssDNA-DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	115.094, 115.094, 300.276
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.300 - 3.460
R-factor	0.2634
Rwork	0.259
R-free	0.30270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.825
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	3.560
High resolution limit [Å]	3.460	3.460
Rmerge	0.137	1.480
Rpim	0.038	0.408
Number of reflections	14675	1434
<I/σ(I)>	9.3	1.3
Completeness [%]	92.5
Redundancy	10.1
CC(1/2)	0.999	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	1.5 M ammonium sulphate, 0.1 M Tris pH 7.5