8TN8
Crystal structure of the murine astrovirus capsid spike at 1.75 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.328, 48.872, 65.463 |
| Unit cell angles | 93.34, 102.91, 114.70 |
Refinement procedure
| Resolution | 43.750 - 1.750 |
| R-factor | 0.1721 |
| Rwork | 0.171 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.819 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.760 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 46845 | 2226 |
| <I/σ(I)> | 14.4 | 1.9 |
| Completeness [%] | 94.8 | 92.52 |
| Redundancy | 1.7 | 1.7 |
| CC(1/2) | 0.995 | 0.936 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | Potassium formate, PEG 3350 |
