8TLY
Human ASCC1 Phosphodiesterase/Ligase Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.879, 62.105, 136.222 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.730 - 2.800 |
R-factor | 0.2086 |
Rwork | 0.206 |
R-free | 0.25560 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.495 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.730 | 2.970 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 12679 | 1990 |
<I/σ(I)> | 9.71 | |
Completeness [%] | 99.6 | |
Redundancy | 1.72 | |
CC(1/2) | 0.988 | 0.796 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 12.5% MPEG 2K, 200 mM imidizole/malate (I/M) pH 5.0, 5% saturated KCl at room temperature. For cryoprotection, 25% ethylene glycol added to the mother liquor |