8TLO

Crystal Structure Analysis of BCL11A in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	116.740, 60.870, 78.350
Unit cell angles	90.00, 108.20, 90.00

Refinement procedure

Resolution	55.450 - 2.760
R-factor	0.2529
Rwork	0.252
R-free	0.26860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.825
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.450	2.859
High resolution limit [Å]	2.760	2.760
Number of reflections	13593	1330
<I/σ(I)>	8.35
Completeness [%]	99.9
Redundancy	8.6	3.6
CC(1/2)	0.990	0.401

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	30% PEG6K and 0.1M Na Citrate pH 5.0