8TLK

CDCA7 (Human) Binds Non-B-form 32-mer DNA oligo Containing a 5mC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.99999
Spacegroup name	C 1 2 1
Unit cell lengths	173.479, 31.538, 94.566
Unit cell angles	90.00, 110.18, 90.00

Refinement procedure

Resolution	43.060 - 2.990
R-factor	0.2454
Rwork	0.243
R-free	0.30180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.487
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.060	3.090
High resolution limit [Å]	2.990	2.990
Rmerge	0.123	0.449
Rpim	0.070	0.333
Number of reflections	8135	772
<I/σ(I)>	6.1	1.7
Completeness [%]	79.7	75.8
Redundancy	3.6	2.3
CC(1/2)	1.000	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2M MgCl2, 0.1 M Bis-Tris pH6.0, 25%PEG3350