8TL1
Structure of Orthoreovirus RNA Chaperone SigmaNS N17
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2021-05-05 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.522, 76.801, 89.090 |
| Unit cell angles | 90.00, 94.27, 90.00 |
Refinement procedure
| Resolution | 88.840 - 3.160 |
| R-factor | 0.21096 |
| Rwork | 0.208 |
| R-free | 0.25110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.460 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.840 | 3.200 |
| High resolution limit [Å] | 3.160 | 3.200 |
| Rmerge | 0.132 | 0.130 |
| Rpim | 0.130 | |
| Number of reflections | 7841 | 302 |
| <I/σ(I)> | 10.09 | |
| Completeness [%] | 86.4 | |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.2% colic acid derivativemix, 0.1 M buffer system 3 (8.5), 30% precipitant Mix3 (Molecular dimensions) |
