8TJH
TGP-E, extreme thermostable green fluorescent protein (TGP) with Q66E mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-04 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.736, 49.064, 59.108 |
| Unit cell angles | 78.55, 72.08, 71.41 |
Refinement procedure
| Resolution | 20.420 - 2.000 |
| R-factor | 0.172 |
| Rwork | 0.169 |
| R-free | 0.22870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.170 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.420 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.183 | 0.783 |
| Rmeas | 0.223 | |
| Rpim | 0.125 | 0.554 |
| Number of reflections | 26322 | 1957 |
| <I/σ(I)> | 7.6 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.7 | 5.4 |
| CC(1/2) | 0.990 | 0.776 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295.15 | 0.35 M magnesium chloride hexahydrate, 0.1 M Tris hydrochloride pH 8.5, 37.5 % PEG 4000 |
