8TJ9

CRYSTAL STRUCTURE OF THE A/Michigan/15/2014(H3N2) INFLUENZA VIRUS HEMAGGLUTININ WITH HUMAN RECEPTOR ANALOG 6'-SLNLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	H 3 2
Unit cell lengths	100.591, 100.591, 394.890
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.530 - 1.900
R-factor	0.1619
Rwork	0.161
R-free	0.18540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.995
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.530	1.960
High resolution limit [Å]	1.900	1.900
Rpim	0.020	0.230
Number of reflections	61131	5474
<I/σ(I)>	42.3	3
Completeness [%]	99.9
Redundancy	18.8
CC(1/2)	0.999	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	40% PEG-400, 0.2 M Lithium sulfate, and 0.1M Tris pH 8.5