8TI4

monovalent bispecific IgG antibodies through novel electrostatic steering mutations at the CH1-CL interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	71.269, 78.815, 80.925
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.413 - 2.100
Rwork	0.201
R-free	0.25140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.379
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.413	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	96578	16522
<I/σ(I)>	4.8
Completeness [%]	89.4
Redundancy	3.8
CC(1/2)	0.989	0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M Bis-Tris at pH 6.5, 25% w/v PEG 3350