8TI4
monovalent bispecific IgG antibodies through novel electrostatic steering mutations at the CH1-CL interface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.269, 78.815, 80.925 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.413 - 2.100 |
| Rwork | 0.201 |
| R-free | 0.25140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.379 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.413 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 96578 | 16522 |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 89.4 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.989 | 0.662 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M Bis-Tris at pH 6.5, 25% w/v PEG 3350 |
