8TH6
Crystal Structure of the G3BP1 NTF2-like domain bound to USP10 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.390, 78.380, 73.410 |
Unit cell angles | 90.00, 108.63, 90.00 |
Refinement procedure
Resolution | 45.200 - 2.340 |
R-factor | 0.21698 |
Rwork | 0.213 |
R-free | 0.29535 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.215 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.210 | 2.420 |
High resolution limit [Å] | 2.340 | 2.340 |
Number of reflections | 15966 | 177 |
<I/σ(I)> | 9.2 | |
Completeness [%] | 63.1 | |
Redundancy | 3.3 | |
CC(1/2) | 0.918 | 0.848 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 0.1 M HEPES pH 7.5, 25% PEG3350 |