8TGP
Crystal structure of SIRT2 with FAM-PEG4-H4K16(myristoyl) peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2021-06-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.138, 76.995, 56.005 |
| Unit cell angles | 90.00, 97.89, 90.00 |
Refinement procedure
| Resolution | 45.010 - 1.760 |
| R-factor | 0.1933 |
| Rwork | 0.190 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.010 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.032 | |
| Rpim | 0.023 | |
| Number of reflections | 29902 | 1339 |
| <I/σ(I)> | 25.5 | |
| Completeness [%] | 96.0 | 86.4 |
| Redundancy | 3.2 | 3 |
| CC(1/2) | 0.996 | 0.923 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M MES monohydrate pH 6.5 (NaOH), 20% w/v PEG 4,000 |
