8TGF

Crystal structure of cEPG5 LIR/LGG-1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	33.991, 37.952, 45.177
Unit cell angles	90.00, 100.10, 90.00

Refinement procedure

Resolution	44.480 - 1.600
R-factor	0.1762
Rwork	0.174
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5azf
RMSD bond length	0.010
RMSD bond angle	1.090
Data reduction software	DIALS
Data scaling software	Aimless (0.7.9)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.480	1.657
High resolution limit [Å]	1.600	1.600
Rmerge	0.036	0.116
Number of reflections	14650	1406
<I/σ(I)>	25.12
Completeness [%]	96.9
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	0.1 M sodium acetate pH 4.6, 21% (w/v) PEG 6000