8TGE

Crystal structure of the Methanosarcina mazei glutamine synthetase in complex with GlnK1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	I 1 2 1
Unit cell lengths	137.990, 178.030, 169.048
Unit cell angles	90.00, 90.36, 90.00

Refinement procedure

Resolution	89.020 - 2.300
R-factor	0.3
Rwork	0.165
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.936
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	BALBES
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.020	2.360
High resolution limit [Å]	2.300	2.300
Rpim	0.068	0.447
Number of reflections	180295	13618
<I/σ(I)>	8.3	1.8
Completeness [%]	99.6
Redundancy	3.1
CC(1/2)	0.994	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		273	PEG 400, 0.1 M MES pH 6.5