8TG7

Structure of Red beta C-terminal domain in complex with SSB C-terminal peptide, Form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	38.313, 69.222, 70.536
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.405 - 1.775
Rwork	0.182
R-free	0.23410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.472
Data reduction software	autoPROC (1.1.7)
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP (11..4.03)
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.410	1.891
High resolution limit [Å]	1.775	1.775
Rmerge	0.055	1.399
Rmeas	0.060	1.516
Rpim	0.024	0.576
Number of reflections	16243	812
<I/σ(I)>	15.1	1.3
Completeness [%]	86.5	25.5
Redundancy	6.3	6.8
CC(1/2)	0.999	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	298	2.0 M ammonium sulfate, 0.1 M Tris pH 8.6