8TG6

tRNA 2'-phosphotransferase (Tpt1) from Aeropyrum pernix in complex with sulfate anions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	H 3 2
Unit cell lengths	101.679, 101.679, 101.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	66.410 - 1.600
R-factor	0.1449
Rwork	0.143
R-free	0.18180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wfx
RMSD bond length	0.012
RMSD bond angle	1.299
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.410	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.063	0.623
Rpim	0.016	0.156
Number of reflections	26676	1302
<I/σ(I)>	31.8	4.9
Completeness [%]	100.0	99.8
Redundancy	17	17
CC(1/2)	1.000	0.952

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M HEPES-Na, pH 7.5, 1.0 M ammonium sulfate, 2% PEG-400