8TG4

tRNA 2'-phosphotransferase (Tpt1) from Aeropyrum pernix in complex with ADP-ribose-2"-phosphate and 2'-OH RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	39.844, 55.701, 42.249
Unit cell angles	90.00, 97.79, 90.00

Refinement procedure

Resolution	41.860 - 1.370
R-factor	0.1499
Rwork	0.149
R-free	0.17500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wfx
RMSD bond length	0.009
RMSD bond angle	1.217
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.860	1.390
High resolution limit [Å]	1.370	1.370
Rmerge	0.037	0.777
Rpim	0.016	0.337
Number of reflections	38249	1848
<I/σ(I)>	22.4	1.9
Completeness [%]	99.3
Redundancy	6.3
CC(1/2)	1.000	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M KNO3, 24% PEG-3350