8TFU
Structure of Red beta C-terminal domain in complex with SSB C-terminal peptide, Form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.810, 68.252, 70.888 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.149 - 1.482 |
| Rwork | 0.181 |
| R-free | 0.22020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.584 |
| Data reduction software | autoPROC (1.1.7) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP (11.4.03) |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.444 | 1.590 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.062 | 1.217 |
| Rmeas | 1.330 | |
| Rpim | 0.526 | |
| Number of reflections | 25018 | 1251 |
| <I/σ(I)> | 14.1 | 1.2 |
| Completeness [%] | 80.1 | 21.2 |
| Redundancy | 6.2 | 6.1 |
| CC(1/2) | 0.409 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.48 M sodium phosphate monobasic monohydrate, 1.2 M potassium phosphate dibasic |
