8TFE
Crystal structure of Fab fragment of anti-HCV E2 antibody (CBH-7)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.935, 101.912, 109.936 |
Unit cell angles | 90.00, 100.24, 90.00 |
Refinement procedure
Resolution | 46.140 - 1.620 |
R-factor | 0.17881 |
Rwork | 0.177 |
R-free | 0.21384 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6x9x |
RMSD bond length | 0.011 |
RMSD bond angle | 1.649 |
Data reduction software | HKL-3000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.650 |
High resolution limit [Å] | 1.620 | 1.620 |
Rmerge | 0.090 | 0.544 |
Rmeas | 0.098 | 0.595 |
Rpim | 0.038 | 0.238 |
Number of reflections | 107383 | 5314 |
<I/σ(I)> | 27.75 | |
Completeness [%] | 98.4 | 98.5 |
Redundancy | 6.4 | 5.6 |
CC(1/2) | 0.981 | 0.911 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1M Sodium phosphate dibasic pH 4.2, 0.2 M NaCl, and 20% PEG8000 |