8TFD

Crystal structure of a stem-loop DNA aptamer complexed with SARS-CoV-2 nucleocapsid protein RNA-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-13
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	37.795, 54.133, 98.813
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.480 - 1.550
R-factor	0.1606
Rwork	0.159
R-free	0.18870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.413
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.130	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.061	0.416
Rmeas	0.066	0.519
Rpim	0.025	0.304
Total number of observations	171070	1371
Number of reflections	27808	636
<I/σ(I)>	16.6	1.3
Completeness [%]	92.1
Redundancy	6.2	2.2
CC(1/2)	0.998	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2M Ammonium formate,10% w/v Polyvinylpyrrolidone, 20% w/v PEG 4000