8TEE
Crystal structure of Kindlin2 in complex with K794Q mutated beta1 integrin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-12 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.91975 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.971, 98.291, 76.003 |
Unit cell angles | 90.00, 96.42, 90.00 |
Refinement procedure
Resolution | 29.940 - 2.490 |
R-factor | 0.2298 |
Rwork | 0.227 |
R-free | 0.27470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xpy |
RMSD bond length | 0.003 |
RMSD bond angle | 0.569 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.940 | 2.550 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.124 | 0.623 |
Number of reflections | 36657 | 2159 |
<I/σ(I)> | 8.6 | 1.8 |
Completeness [%] | 97.4 | 77.5 |
Redundancy | 6.8 | 6.2 |
CC(1/2) | 0.990 | 0.875 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris, pH 8.5, 10% isopropanal |