8TEE

Crystal structure of Kindlin2 in complex with K794Q mutated beta1 integrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.91975
Spacegroup name	P 1 21 1
Unit cell lengths	72.971, 98.291, 76.003
Unit cell angles	90.00, 96.42, 90.00

Refinement procedure

Resolution	29.940 - 2.490
R-factor	0.2298
Rwork	0.227
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xpy
RMSD bond length	0.003
RMSD bond angle	0.569
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.940	2.550
High resolution limit [Å]	2.490	2.490
Rmerge	0.124	0.623
Number of reflections	36657	2159
<I/σ(I)>	8.6	1.8
Completeness [%]	97.4	77.5
Redundancy	6.8	6.2
CC(1/2)	0.990	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Tris, pH 8.5, 10% isopropanal