8TEC
Crystal structure of Kindlin2 in complex with acylated beta1 integrin peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.487, 102.093, 76.029 |
Unit cell angles | 90.00, 96.81, 90.00 |
Refinement procedure
Resolution | 75.490 - 2.040 |
R-factor | 0.1955 |
Rwork | 0.194 |
R-free | 0.23020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xpy |
RMSD bond length | 0.007 |
RMSD bond angle | 0.812 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 102.090 | 2.090 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.069 | 1.728 |
Rmeas | 0.075 | |
Rpim | 0.028 | |
Number of reflections | 69860 | 3942 |
<I/σ(I)> | 12.8 | 0.9 |
Completeness [%] | 98.8 | 87.4 |
Redundancy | 6.8 | 6.1 |
CC(1/2) | 0.999 | 0.394 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris, pH 8.5, 10% isopropanal |