8TDZ

16mer self-complementary duplex RNA with D:U pair sequence 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2023-04-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.038413
Spacegroup name	H 3 2
Unit cell lengths	41.870, 41.870, 124.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.430 - 1.640
R-factor	0.19
Rwork	0.188
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.002
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.670
High resolution limit [Å]	1.640	1.640
Rmerge	0.053	0.184
Rmeas	0.057	0.204
Rpim	0.019	0.084
Number of reflections	5403	250
<I/σ(I)>	35.5	8.4
Completeness [%]	99.6	100
Redundancy	8.7	5
CC(1/2)		0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	40 mM Lithium chloride, 20 mM Magnesium chloride hexahydrate, 40 mM Sodium cacodylate trihydrate pH 5.5, 30% v/v (+/-)-2-Methyl-2,4-pentanediol, 2 mM Hexammine cobalt(III) chloride