8TDZ
16mer self-complementary duplex RNA with D:U pair sequence 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 2.0.1 |
Synchrotron site | ALS |
Beamline | 2.0.1 |
Temperature [K] | 99 |
Detector technology | PIXEL |
Collection date | 2023-04-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.038413 |
Spacegroup name | H 3 2 |
Unit cell lengths | 41.870, 41.870, 124.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.430 - 1.640 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.22160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.002 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.670 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.053 | 0.184 |
Rmeas | 0.057 | 0.204 |
Rpim | 0.019 | 0.084 |
Number of reflections | 5403 | 250 |
<I/σ(I)> | 35.5 | 8.4 |
Completeness [%] | 99.6 | 100 |
Redundancy | 8.7 | 5 |
CC(1/2) | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 40 mM Lithium chloride, 20 mM Magnesium chloride hexahydrate, 40 mM Sodium cacodylate trihydrate pH 5.5, 30% v/v (+/-)-2-Methyl-2,4-pentanediol, 2 mM Hexammine cobalt(III) chloride |