8TDY
16mer self-complementary duplex RNA with D:U pair sequence 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 2.0.1 |
| Synchrotron site | ALS |
| Beamline | 2.0.1 |
| Temperature [K] | 99 |
| Detector technology | PIXEL |
| Collection date | 2023-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.038413 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 41.146, 41.146, 125.047 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.680 - 1.660 |
| R-factor | 0.1975 |
| Rwork | 0.196 |
| R-free | 0.22380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.955 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.097 | 0.520 |
| Rmeas | 0.104 | 0.569 |
| Rpim | 0.035 | 0.225 |
| Number of reflections | 5058 | 483 |
| <I/σ(I)> | 4.2 | 4.2 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 8.5 | 6.1 |
| CC(1/2) | 1.000 | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 80 mM Strontium chloride hexahydrate, 20 mM Magnesium chloride hexahydrate, 40 mM Sodium cacodylate trihydrate pH 7.0, 20% v/v (+/-)-2-Methyl-2,4-pentanediol, 12 mM Spermine |
