8TCE
Lipoprotein(a) Kringle IV domain 8 - Lp(a) KIV8 in complex with LY3353871
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.602, 57.574, 38.305 |
Unit cell angles | 90.00, 94.70, 90.00 |
Refinement procedure
Resolution | 18.740 - 1.070 |
R-factor | 0.16214 |
Rwork | 0.161 |
R-free | 0.18291 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.379 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.740 | 1.130 |
High resolution limit [Å] | 1.070 | 1.070 |
Rmerge | 0.068 | 0.607 |
Number of reflections | 61826 | 5622 |
<I/σ(I)> | 7.9 | 1.8 |
Completeness [%] | 91.1 | 91.1 |
Redundancy | 3.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1000 mM trisodium citrate pH 7.0, 100 mM HEPES pH 6.0 |