8TCB
Structure of human C4b-binding protein alpha chain CCP domains 1 and 2 in complex with the hypervariable region of group A Streptococcus M87 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 43.176, 73.802, 190.398 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.810 - 2.690 |
| R-factor | 0.2375 |
| Rwork | 0.235 |
| R-free | 0.27840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.619 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.810 | 2.820 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.090 | 0.953 |
| Rmeas | 0.097 | 1.155 |
| Rpim | 0.037 | 0.641 |
| Number of reflections | 17478 | 2045 |
| <I/σ(I)> | 20.3 | 1.4 |
| Completeness [%] | 98.7 | 90.1 |
| Redundancy | 6.8 | 3 |
| CC(1/2) | 0.997 | 0.452 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium citrate, pH 4.8, 0.2 M KH2PO4, 25% w/v PEG 3350 |
