8TC8

Human asparaginyl-tRNA synthetase bound to adenosine 5'-sulfamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	115.851, 126.285, 160.598
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.290 - 1.900
R-factor	0.1827
Rwork	0.181
R-free	0.21720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xix
RMSD bond length	0.007
RMSD bond angle	0.860
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.290	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.163	2.601
Rmeas	0.192	3.070
Rpim	0.072	1.186
Number of reflections	185028	9037
<I/σ(I)>	7.5
Completeness [%]	99.9
Redundancy	6.9
CC(1/2)	0.998	0.502

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20% (v/v) glycerol, 40 mM potassium phosphate and 14% polyethylene glycol 8,000, and 100-mM Tris pH 7.6