8TBI

Tricomplex of RMC-7977, NRAS WT, and CypA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95371
Spacegroup name	P 21 21 21
Unit cell lengths	65.490, 84.270, 126.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.860 - 1.590
R-factor	0.182
Rwork	0.181
R-free	0.20780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.697
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.860	1.647
High resolution limit [Å]	1.590	1.590
Rmerge	0.119	2.356
Rmeas	0.129	2.524
Rpim	0.047	0.898
Number of reflections	94249	9290
<I/σ(I)>	7.4	0.77
Completeness [%]	99.9	99.91
Redundancy	7.4	7.7
CC(1/2)	0.998	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	27% PEG4000, 0.1 M Tris-HCl, pH 8.0