8TBG

Tricomplex of RMC-7977, HRAS WT, and CypA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95371
Spacegroup name	P 21 21 21
Unit cell lengths	65.450, 80.860, 128.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.290 - 1.200
R-factor	0.1622
Rwork	0.161
R-free	0.19180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.896
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.290	1.243
High resolution limit [Å]	1.200	1.200
Rmerge	0.097	0.725
Rmeas	0.105	1.828
Rpim	0.039	0.811
Number of reflections	211361	20454
<I/σ(I)>	8.22	0.82
Completeness [%]	99.5	97.18
Redundancy	7.1	4.8
CC(1/2)	0.998	0.357

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	28% PEG4000, 0.1 M Tris, pH 8