8TBF

Tricomplex of RMC-7977, KRAS WT, and CypA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95375
Spacegroup name	P 21 21 21
Unit cell lengths	65.757, 84.240, 125.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.290 - 1.500
R-factor	0.1794
Rwork	0.178
R-free	0.21040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.939
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.290	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.059	0.389
Rmeas	0.063	0.418
Rpim	0.023	0.151
Number of reflections	112271	11048
<I/σ(I)>	17.1	4.54
Completeness [%]	99.8	99.57
Redundancy	7.3	7.4
CC(1/2)	0.997	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	24% PEG4000, 0.1 M Tris-HCl, pH 8.0