8T8S
Sortilin-PGRN peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999982608809 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 237.030, 237.030, 89.427 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 205.270 - 2.990 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.453 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 205.270 | 3.240 |
High resolution limit [Å] | 2.990 | 2.990 |
Rmerge | 0.093 | 0.450 |
Number of reflections | 58113 | 12291 |
<I/σ(I)> | 10.87 | |
Completeness [%] | 99.4 | 99.2 |
Redundancy | 3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 0.10 M Tris, 30.00 %w/v PEG 3000, 0.20 M NaCl |