8T8B

Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site formyl-MAI-tripeptidyl-tRNA analog ACCA-IAMf at 2.65A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	207.110, 441.130, 612.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	119.320 - 2.650
R-factor	0.2129
Rwork	0.211
R-free	0.25690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rqb
RMSD bond length	0.004
RMSD bond angle	0.845
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	119.320	119.320	2.720
High resolution limit [Å]	2.650	11.850	2.650
Rmerge	0.190	0.047	1.939
Rmeas	0.207	0.051	2.138
Total number of observations	9752234
Number of reflections	1585401	18710	116896
<I/σ(I)>	8.02	30.16	1.02
Completeness [%]	99.0	98.7	99.1
Redundancy	6.151	6.797	5.725
CC(1/2)	0.995	0.997	0.169

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME