8T73

Crystal structure of KRAS4a-R151G with bound GDP and Mg ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 3
Unit cell lengths	84.779, 84.779, 41.735
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.390 - 1.500
R-factor	0.1555
Rwork	0.154
R-free	0.18790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.142
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.390	42.390	1.590
High resolution limit [Å]	1.500	4.470	1.500
Rmerge	0.062	0.043	0.463
Rmeas	0.067	0.047	0.518
Number of reflections	52617	2030	7326
<I/σ(I)>	17.94
Completeness [%]	97.3
Redundancy	6.5
CC(1/2)	0.999	0.998	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM HEPES pH 7.4, 2 M ammonium sulfate , 0.1 M MgCl2.6H2O