8T71

Crystal Structure of WT KRAS4a with bound GDP and Mg ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	132.893, 34.534, 94.838
Unit cell angles	90.00, 120.60, 90.00

Refinement procedure

Resolution	46.130 - 1.800
R-factor	0.18
Rwork	0.178
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.130	46.130	1.910
High resolution limit [Å]	1.800	5.360	1.800
Rmerge	0.111	0.040	1.669
Rmeas	0.120	0.043	1.806
Number of reflections	34137	1369	5431
<I/σ(I)>	11.57
Completeness [%]	97.2
Redundancy	6.8
CC(1/2)	0.998	0.999	0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium iodide, 2.2 M ammonium sulfate