8T6E
Crystal structure of T33-28.3: Deep-learning sequence design of co-assembling tetrahedron protein nanoparticles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 1 |
| Unit cell lengths | 113.737, 114.050, 114.197 |
| Unit cell angles | 62.09, 77.86, 89.41 |
Refinement procedure
| Resolution | 48.470 - 2.480 |
| R-factor | 0.2052 |
| Rwork | 0.205 |
| R-free | 0.23980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.446 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.470 | 2.520 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.062 | 0.630 |
| Number of reflections | 171459 | 8425 |
| <I/σ(I)> | 6.3 | 0.7 |
| Completeness [%] | 98.1 | 97.5 |
| Redundancy | 2.4 | 2.2 |
| CC(1/2) | 0.994 | 0.341 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.26 M sodium phosphate, 0.14 M potassium phosphate |
