8T6C
Crystal structure of T33-18.2: Deep-learning sequence design of co-assembling tetrahedron protein nanoparticles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-05-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 138.812, 138.812, 129.106 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.870 - 1.920 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.618 |
| Data reduction software | DIALS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.870 | 1.960 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 70870 | 2619 |
| <I/σ(I)> | 9.9 | 0.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.7 |
| CC(1/2) | 0.997 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.9 M NPS, 0.1 M Tris-Bicine, pH 8.5, 30% v/v glycerol + PEG4000 |
