8T5J

Crystal structure of outer membrane lipoprotein carrier protein (LolA) from Francisella philomiragia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-08
Detector	Bruker PHOTON III
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	61.850, 61.850, 86.611
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.100 - 1.550
R-factor	0.1723
Rwork	0.170
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.962
Data reduction software	SAINT
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_4933: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.170	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.097	2.648
Rmeas	0.099	2.730
Rpim	0.020	0.662
Total number of observations	718556	23447
Number of reflections	28420	1382
<I/σ(I)>	24.7	1.6
Completeness [%]	100.0
Redundancy	25.3	17
CC(1/2)	1.000	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	Berkeley D10: 20% (w/v) PEG 3350, 100 mM Hepes pH 7.5, 100 mM Lithium sulfate, 100 mM Magnesium chloride, 10% (w/v) 1,6-Hexanediol. FrphA.20731.a.VL2.PB00125 at 14.5 mg/mL. plate 13468 well D10 drop1, Cryo: 80% crystallant+20% PEG200