8T59

Crystal structure of Para.09 bound to TREM2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-14
Detector	DECTRIS EIGER X 1M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	54.170, 68.160, 254.796
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.780 - 2.000
R-factor	0.2015
Rwork	0.200
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.923
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.780	2.071
High resolution limit [Å]	2.000	2.000
Number of reflections	64863	6409
<I/σ(I)>	13.08
Completeness [%]	99.9
Redundancy	6.3
CC(1/2)	0.998	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES pH6.0, 10 mM ZnCl2, 20% PEG6000, 3% 1,6-hexandiol