8T55

Co-crystal structure of the WD-repeat domain of human WDR91 in complex with MR46654

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-15
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	76.696, 121.618, 132.027
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.780 - 2.200
R-factor	0.2455
Rwork	0.245
R-free	0.28777
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.270
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.080	0.052	1.014
Rmeas	0.086	0.056	1.083
Rpim	0.032	0.020	0.375
Total number of observations	478836
Number of reflections	62021	3219	3075
<I/σ(I)>	6.2
Completeness [%]	97.9	94.2	98.4
Redundancy	7.7	7.2	8.2
CC(1/2)	0.998	0.999	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	12% (w/v) PEG3350, 0.2 M KCl, 0.1 M MES pH 6.5